logo
Loading...
Design of 8-mer peptides that block *Clostridioides difficile* toxin A in intestinal cells
Medicine and HealthCommunications Biology

Design of 8-mer peptides that block *Clostridioides difficile* toxin A in intestinal cells

S. Sarma, C. M. Catella, et al.

Discover groundbreaking research conducted by Sudeep Sarma, Carly M. Catella, and their team, focusing on peptide inhibitors that effectively block Toxin A of *Clostridioides difficile* in human colon epithelial cells. Their identified peptide, SA1, shows promising binding affinity, opening avenues for innovative treatments against a pressing global health issue.... show more
GeneralSummaryMetrics
Abstract
Infections by Clostridioides difficile, a bacterium that targets the large intestine, are mediated by two exotoxins, toxins A and B. Short peptides that can be delivered to the gut and inhibit the catalytic activity of these toxins represent a promising therapeutic strategy. This study combines a Peptide Binding Design (PepBD) algorithm, molecular simulations, a rapid bead-based assay for peptide:toxin binding, a primary human colon epithelial cell assay, and surface plasmon resonance (SPR) measurements to develop peptide inhibitors that block Toxin A in intestinal epithelial cells. One peptide, SA1, blocks TcdA toxicity in primary-derived human colon epithelial cells and binds TcdA with a KD of 56.1 ± 29.8 nM by SPR.
Publisher
Communications Biology
Published On
Aug 26, 2023
Authors
Sudeep Sarma, Carly M. Catella, Ellyce T. San Pedro, Xingqing Xiao, Deniz Durmusoglu, Stefano Menegatti, Nathan Crook, Scott T. Magness, Carol K. Hall
DOI
https://doi.org/10.1038/s42003-023-05242-x
Tags
Clostridioides difficileToxin Apeptide inhibitorshuman colon epithelial cellsbinding affinity
Related Publications

Explore these studies to deepen your understanding

Adjacent work that informs or extends this paper's methodology and findings.

Development of a novel testis-on-a-chip that demonstrates reciprocal crosstalk between Sertoli and Leydig cells in testicular tissue
Medicine and Health

Development of a novel testis-on-a-chip that demonstrates reciprocal crosstalk between Sertoli and Leydig cells in testicular tissue

S. Park, M. G. Kook, et al.

MFRP is a molecular hub that organizes the apical membrane of RPE cells by engaging in interactions with specific proteins and lipids
Medicine and Health

MFRP is a molecular hub that organizes the apical membrane of RPE cells by engaging in interactions with specific proteins and lipids

A. Tworak, R. Smidak, et al.

A type I interferon footprint in pre-operative biopsies is an independent biomarker that in combination with CD8+ T cell quantification can improve the prediction of response to neoadjuvant treatment of rectal adenocarcinoma
Medicine and Health

A type I interferon footprint in pre-operative biopsies is an independent biomarker that in combination with CD8+ T cell quantification can improve the prediction of response to neoadjuvant treatment of rectal adenocarcinoma

A. Rezapour, D. Rydbeck, et al.

Dietary prebiotics promote intestinal *Prevotella* in association with a low-responding phenotype in a murine oxazolone-induced model of atopic dermatitis
Veterinary Science

Dietary prebiotics promote intestinal *Prevotella* in association with a low-responding phenotype in a murine oxazolone-induced model of atopic dermatitis

A. Laigaard, L. Krych, et al.

Listen, Learn & Level Up
Over 10,000 hours of research content in 25+ fields, available in 22+ languages.
No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.
listen to research audio papers with researchbunny