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Multiplexed fluorescence and scatter detection with single cell resolution using on-chip fiber optics for droplet microfluidic applications

Engineering and Technology

Multiplexed fluorescence and scatter detection with single cell resolution using on-chip fiber optics for droplet microfluidic applications

P. Gupta, A. Mohan, et al.

Discover OptiDrop, a groundbreaking optofluidic platform designed for multiparametric optical sensing of droplets in microfluidic applications. This innovative approach utilizes on-chip optical fibers for single-cell resolution detection of biomolecules, presenting a powerful, cost-effective alternative to traditional microscopy techniques. This research, conducted by Preksha Gupta and team, sheds light on MHC proteins and their responsiveness to IFNγ stimulation.

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Playback language: English
Abstract
This paper introduces OptiDrop, a novel optofluidic platform for multiparametric optical sensing of droplets in microfluidic applications. OptiDrop uses on-chip optical fibers to detect scattered and fluorescent signals from droplets with single-cell resolution, offering a simplified, miniaturized, and cost-effective alternative to microscopy-based methods. The platform's capabilities are demonstrated through a differential expression analysis of MHC proteins in response to IFNγ stimulation, showcasing its sensitivity in detecting cell surface biomarkers.
Publisher
Microsystems & Nanoengineering
Published On
Jan 28, 2024
Authors
Preksha Gupta, Ambili Mohan, Apurv Mishra, Atindra Nair, Neeladri Chowdhury, Dhanush Balekai, Kavyashree Rai, Anil Prabhakar, Taslimarif Saiyed
Tags
OptiDrop
optofluidics
optical sensing
multiparametric analysis
microfluidics
MHC proteins
single-cell resolution

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