Environmental Studies and Forestry
Evaluation of the Impact of Concentration and Extraction Methods on the Targeted Sequencing of Human Viruses from Wastewater
M. Jiang, A. L. W. Wang, et al.
Sequencing human viruses in wastewater faces low-abundance challenges; this study compared four concentration/extraction methods (Innovaprep, Nanotrap, Promega, Solids) and found method-dependent virus profiles. Innovaprep ultrafiltration yielded the highest sequencing sensitivity and assembled near-complete genomes, while Promega and Nanotrap were more sensitive for SARS-CoV-2 by dPCR. Research was conducted by Authors present in <Authors> tag.
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