Medicine and Health

Single-cell sequencing of ascites fluid illustrates heterogeneity and therapy-induced evolution during gastric cancer peritoneal metastasis

X. Huang, M. Pang, et al.

This fascinating study delves into the complexities of peritoneal metastasis in gastric cancer, unveiling how certain immune cells evolve under therapy and their impact on prognosis. Conducted by a dedicated team of researchers, these insights shed light on the trajectories of cancer and immune cells during metastasis progression, promising innovative pathways for therapy-resistant cases.

00:00

Playback language: English

Index

Abstract

Peritoneal metastasis is a leading cause of death in gastrointestinal cancers. This study characterizes single-cell transcriptomes of ascites cancer/immune cells from 35 gastric cancer patients, revealing increased pro-angiogenic monocyte-like dendritic cells with reduced antigen-presenting capacity, correlating with poor prognosis. Therapy-induced evolution of these cells and regulatory/proliferative T cells is described, along with high-plasticity gastric cancer clusters prone to shifting to a high-proliferative phenotype via paligenosis. Autophagy-related genes (MARCKS and TXNIP) mark high-plasticity cancer with poor prognosis, and autophagy inhibitors induce apoptosis in patient-derived organoids. These findings offer insights into cancer/immune cell trajectories underlying metastasis progression and therapy resistance.

Publisher

Nature Communications

Published On

Feb 14, 2023

Authors

Xuan-Zhang Huang, Min-Jiao Pang, Jia-Yi Li, Han-Yu Chen, Jing-Xu Sun, Yong-Xi Song, Hong-Jie Ni, Shi-Yu Ye, Shi Bai, Teng-Hui Li, Xin-Yu Wang, Jing-Yuan Lu, Jin-Jia Yang, Xun Sun, Jason C. Mills, Zhi-Feng Miao, Zhen-Ning Wang

Related Publications

Explore these studies to deepen your understanding of the subject.

Veterinary Science

Single-cell RNA sequencing reveals the cellular and molecular heterogeneity of treatment-naïve primary osteosarcoma in dogs

D. T. Ammons, L. S. Hopkins, et al.

Medicine and Health

Single-cell sequencing unveils key contributions of immune cell populations in cancer-associated adipose wasting

J. Han, Y. Wang, et al.

Medicine and Health

speedingCARs: accelerating the engineering of CAR T cells by signaling domain shuffling and single-cell sequencing

R. Castellanos-rueda, R. B. D. Roberto, et al.

Biology

Biology of Circulating Tumor Cells through Single-Cell RNA Sequencing: Implications for Precision Medicine in Cancer

S. Orrapin, P. Thongkumkoon, et al.

Listen, Learn & Level Up

Over 10,000 hours of research content in 25+ fields, available in 12+ languages.

No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.

listen to research audio papers with researchbunny