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Single-cell RNA sequencing reveals the cellular and molecular heterogeneity of treatment-naïve primary osteosarcoma in dogs

Veterinary Science

Single-cell RNA sequencing reveals the cellular and molecular heterogeneity of treatment-naïve primary osteosarcoma in dogs

D. T. Ammons, L. S. Hopkins, et al.

Discover groundbreaking insights into osteosarcoma, an aggressive bone cancer, through single-cell RNA sequencing in treatment-naïve dogs. This research, conducted by Dylan T. Ammons, Leone S. Hopkins, Kathryn E. Cronise, Jade Kurihara, Daniel P. Regan, and Steven Dow, reveals 41 distinct cell types and underscores the translational potential of canine models in immuno-oncology.

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Playback language: English
Abstract
Osteosarcoma (OS) is a heterogeneous and aggressive bone cancer. This study used single-cell RNA sequencing (scRNA-seq) to analyze the tumor microenvironment (TME) in six treatment-naïve dogs with spontaneously occurring primary OS. Analysis of 35,310 cells identified 41 distinct cell types, including follicular helper T cells, mature regulatory dendritic cells (mregDCs), and eight tumor-associated macrophage (TAM) populations. Cell-cell interaction analysis suggested key roles for mregDCs and TAMs in modulating T cell immunity. Cross-species analysis showed high similarity between human and canine OS, making canine OS a valuable translational research model for immuno-oncology.
Publisher
Communications Biology
Published On
Apr 24, 2024
Authors
Dylan T. Ammons, Leone S. Hopkins, Kathryn E. Cronise, Jade Kurihara, Daniel P. Regan, Steven Dow
Tags
osteosarcoma
tumor microenvironment
single-cell RNA sequencing
cell types
immuno-oncology
canine model
T cell immunity

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