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Single-cell RNA sequencing reveals the cellular and molecular heterogeneity of treatment-naïve primary osteosarcoma in dogs

Veterinary Science

Single-cell RNA sequencing reveals the cellular and molecular heterogeneity of treatment-naïve primary osteosarcoma in dogs

D. T. Ammons, L. S. Hopkins, et al.

Discover groundbreaking insights into osteosarcoma, an aggressive bone cancer, through single-cell RNA sequencing in treatment-naïve dogs. This research, conducted by Dylan T. Ammons, Leone S. Hopkins, Kathryn E. Cronise, Jade Kurihara, Daniel P. Regan, and Steven Dow, reveals 41 distinct cell types and underscores the translational potential of canine models in immuno-oncology.

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~3 min • Beginner • English
Abstract
Osteosarcoma (OS) is a heterogeneous, aggressive malignancy of the bone that disproportionally affects children and adolescents. Therapeutic interventions for OS are limited, which is in part due to the complex tumor microenvironment (TME). As such, we used single-cell RNA sequencing (scRNA-seq) to describe the cellular and molecular composition of the TME in 6 treatment-naïve dogs with spontaneously occurring primary OS. Through analysis of 35,310 cells, we identified 41 transcriptomically distinct cell types including the characterization of follicular helper T cells, mature regulatory dendritic cells (mregDCs), and 8 tumor-associated macrophage (TAM) populations. Cell-cell interaction analysis predicted that mregDCs and TAMs play key roles in modulating T cell mediated immunity. Furthermore, we completed cross-species cell type gene signature homology analysis and found a high degree of similarity between human and canine OS. The data presented here act as a roadmap of canine OS which can be applied to advance translational immuno-oncology research.
Publisher
Communications Biology
Published On
Apr 24, 2024
Authors
Dylan T. Ammons, Leone S. Hopkins, Kathryn E. Cronise, Jade Kurihara, Daniel P. Regan, Steven Dow
Tags
osteosarcoma
tumor microenvironment
single-cell RNA sequencing
cell types
immuno-oncology
canine model
T cell immunity
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