Understanding human development is crucial. This study models early human embryo development using expanded pluripotent stem cells (EPSCs) in 3D. A protocol generated self-organizing cystic structures mimicking pre-implantation development, forming blastocyst-like morphology and transitioning to post-implantation-like organization. Single-cell RNA sequencing revealed cell subsets resembling epiblast, hypoblast, and trophectoderm lineages, though with key differences from natural blastocysts. This stem cell platform provides insights into human embryogenesis but also highlights divergences from natural development.
Publisher
NATURE COMMUNICATIONS
Published On
Sep 21, 2021
Authors
Berna Sozen, Victoria Jorgensen, Bailey A. T. Weatherbee, Sisi Chen, Meng Zhu, Magdalena Zernicka-Goetz
Tags
embryo development
expanded pluripotent stem cells
blastocyst
human embryogenesis
cell lineage
self-organization
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