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Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

Medicine and Health

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

L. Cheng, M. Xie, et al.

This groundbreaking study introduces a two-step, xeno-free method for generating valve endothelial-like cells from human pluripotent stem cells. The research, conducted by a team including LinXi Cheng and MingHui Xie, reveals the pivotal roles of TGFβ1 and BMP4 in cell differentiation, leading to superior performance on decellularized porcine aortic valve scaffolds. Discover how this advancement could reshape tissue engineering for heart valves!

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Playback language: English
Abstract
This study describes a two-step, chemically defined, xeno-free method for generating valve endothelial-like cells (VELs) from human pluripotent stem cells (hPSCs). hPSCs were first differentiated into KDR+/ISL1+ cardiac progenitors (CPCs), then into VELs via an intermediate endocardial cushion cell (ECC) stage. TGFβ1 and BMP4 were key in specifying VEC fate by activating NOTCH/WNT signaling and targets like ATF3 and KLF transcription factors. hPSC-derived VELs, when seeded onto decellularized porcine aortic valve (DCV) scaffolds, showed superior proliferation and clonogenicity compared to primary VECs and HAECs. This method provides efficient generation of hPSC-derived valvular cells for potential use in valve organoids or tissue-engineered heart valves.
Publisher
Communications Biology
Published On
Sep 06, 2021
Authors
LinXi Cheng, MingHui Xie, WeiHua Qiao, Yu Song, YanYong Zhang, YingChao Geng, WeiLin Xu, Lin Wang, Zheng Wang, Kai Huang, NianGuo Dong, YuHua Sun
Tags
valve endothelial-like cells
human pluripotent stem cells
cardiac progenitors
TGFβ1
BMP4
tissue engineering
heart valves

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