This study explores the in vivo application of a 3D-printed tissue-engineered skin substitute using an innovative biomaterial loaded with human adipose-derived stem cells (hADSCs) for wound healing. A novel biomaterial composed of adipose tissue decellularized extracellular matrix (dECM) pre-gel, methacrylated gelatin (GelMA), and methacrylated hyaluronic acid (HAMA) was developed and characterized. hADSCs-loaded skin substitutes were 3D printed and applied to full-thickness skin wounds in nude mice. Results showed accelerated wound healing in the experimental group compared to controls, with reduced inflammation, increased blood perfusion, and enhanced re-epithelialization, collagen deposition, and angiogenesis.
