This study investigated the association between gut microbiota and metabolome composition and colorectal cancer (CRC) development. A novel sampling technique was used to collect mucosa-associated microbiota (MAM) and metabolome from the surface of colon polyps without compromising tissue integrity. The results showed distinct microbial and metabolic signatures in low-grade versus high-grade dysplastic polyps, suggesting a role for specific bacteria and metabolites in CRC initiation and progression. Driver bacteria were enriched in low-grade polyps, while passenger bacteria were enriched in high-grade polyps. Integrated analysis revealed correlations between specific bacterial genera and metabolite classes.

M.G. Clavenna, M. La Vecchia, M. Sculco, S. Joseph, E. Barberis, E. Amede, M. Mellai, S. Brossa, G. Borgonovi, P. Occhipinti, R. Boldorini, E. Robotti, A. Aspesi, I. Degano, A. Aimaretti