Medicine and Health

Library adaptors with integrated reference controls improve the accuracy and reliability of nanopore sequencing

H. M. Gunter, S. E. Youlten, et al.

Discover the revolutionary Control Library Adaptors (CAPTORS) that are set to transform next-generation sequencing accuracy and reliability. Developed by a team of researchers, including Helen M. Gunter and Scott E. Youlten, CAPTORS enhance the sequencing process by integrating encoded information that significantly improves diagnostic capabilities, especially for breast cancer variants.

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Abstract

Library adaptors are short oligonucleotides attached to RNA and DNA samples for next-generation sequencing (NGS). This paper introduces Control Library Adaptors (CAPTORS), which measure NGS accuracy and reliability. Integrated into library preparation, CAPTORS' encoded information is retrieved during sequencing. The authors demonstrate CAPTORS' use in measuring nanopore sequencing accuracy, evaluating metagenomic and RNA sequencing performance, and improving sample normalization. Customization for clinical diagnoses is also shown, correcting systematic sequencing errors and improving breast cancer *BRCA1/2* variant diagnosis. CAPTORS offer a simple and effective method to enhance NGS accuracy and reliability.

Publisher

Nature Communications

Published On

Oct 28, 2022

Authors

Helen M. Gunter, Scott E. Youlten, Bindu Swapna Madala, Andre L. M. Reis, Igor Stevanovski, Ted Wong, Sarah K. Kummerfield, Ira W. Deveson, Nadia S. Santini, Esteban Marcellin, Tim R. Mercer

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