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Abstract
This paper presents image-guided, high-throughput mass spectrometry protocols for chemically profiling dense-core vesicles (DCVs) and lucent vesicles (LVs). The method reveals chemical heterogeneity within and between vesicle populations, identifying distinct DCV subtypes based on peptide content and differences in lipid composition between DCVs and LVs. The approach is adaptable to various subcellular structures.
Publisher
Nature Methods
Published On
Sep 30, 2021
Authors
Daniel C. Castro, Yuxuan Richard Xie, Stanislav S. Rubakhin, Elena V. Romanova, Jonathan V. Sweedler
Tags
dense-core vesicles
lucent vesicles
mass spectrometry
chemical profiling
subcellular structures

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