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Image-guided MALDI mass spectrometry for high-throughput single-organelle characterization

Biology

Image-guided MALDI mass spectrometry for high-throughput single-organelle characterization

D. C. Castro, Y. R. Xie, et al.

Discover groundbreaking protocols for image-guided, high-throughput mass spectrometry that intricately profile chemical variations in dense-core and lucent vesicles. This innovative research, conducted by Daniel C. Castro, Yuxuan Richard Xie, Stanislav S. Rubakhin, Elena V. Romanova, and Jonathan V. Sweedler, uncovers unique subtypes of vesicles and paves the way for deeper understanding of their roles in cell biology.

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Playback language: English
Abstract
This paper presents image-guided, high-throughput mass spectrometry protocols for chemically profiling dense-core vesicles (DCVs) and lucent vesicles (LVs). The method reveals chemical heterogeneity within and between vesicle populations, identifying distinct DCV subtypes based on peptide content and differences in lipid composition between DCVs and LVs. The approach is adaptable to various subcellular structures.
Publisher
Nature Methods
Published On
Sep 30, 2021
Authors
Daniel C. Castro, Yuxuan Richard Xie, Stanislav S. Rubakhin, Elena V. Romanova, Jonathan V. Sweedler
Tags
dense-core vesicles
lucent vesicles
mass spectrometry
chemical profiling
subcellular structures

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