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Abstract
This paper introduces a Spectrum and Polarization Optical Tomography (SPOT) technique for studying subcellular lipidomics in live cells using Nile Red staining. SPOT simultaneously resolves membrane morphology, polarity, and phase from three optical dimensions. The high-throughput optical properties reveal lipid heterogeneities across ten subcellular compartments at different developmental stages. Real-time monitoring of multi-organelle interactive activities during cell division reveals sophisticated lipid dynamics.
Publisher
Nature Communications
Published On
Nov 18, 2020
Authors
Karl Zhanghao, Wenhui Liu, Meiqi Li, Zihan Wu, Xiao Wang, Xingye Chen, Chunyan Shan, Haoqian Wang, Xiaowei Chen, Qionghai Dai, Peng Xi, Dayong Jin
Tags
Spectrum Optical Tomography
lipidomics
live cells
Nile Red staining
membrane morphology
polarization
subcellular compartments

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