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Abstract
Studying neuronal activity at synapses requires high spatiotemporal resolution. This paper develops an efficient adaptive optics (AO) method that corrects distorted wavefronts of Bessel focus, enabling high-spatial and high-temporal resolution imaging at depth. Applying AO Bessel focus scanning two-photon fluorescence microscopy (2PFM) to zebrafish larval and mouse brains, the study demonstrates improvements in sensitivity and resolution of structural and functional measurements of synapses in vivo, enabling volumetric measurements of synaptic calcium and glutamate activity with high accuracy.
Publisher
Nature Communications
Published On
Nov 16, 2021
Authors
Wei Chen, Ryan G. Natan, Yuhan Yang, Shih-Wei Chou, Qinrong Zhang, Ehud Y. Isacoff, Na Ji
Tags
neuronal activity
adaptive optics
two-photon microscopy
synapses
zebrafish
mouse brain
volumetric measurements

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