Biology

In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

W. Chen, R. G. Natan, et al.

Discover groundbreaking advancements in neuronal imaging with an innovative adaptive optics method developed by Wei Chen and collaborators. This research enhances the resolution and sensitivity of synaptic measurements in living organisms, enabling precise volumetric analysis of calcium and glutamate dynamics.

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Abstract

Studying neuronal activity at synapses requires high spatiotemporal resolution. This paper develops an efficient adaptive optics (AO) method that corrects distorted wavefronts of Bessel focus, enabling high-spatial and high-temporal resolution imaging at depth. Applying AO Bessel focus scanning two-photon fluorescence microscopy (2PFM) to zebrafish larval and mouse brains, the study demonstrates improvements in sensitivity and resolution of structural and functional measurements of synapses in vivo, enabling volumetric measurements of synaptic calcium and glutamate activity with high accuracy.

Publisher

Nature Communications

Published On

Nov 16, 2021

Authors

Wei Chen, Ryan G. Natan, Yuhan Yang, Shih-Wei Chou, Qinrong Zhang, Ehud Y. Isacoff, Na Ji

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