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High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

Biology

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

K. Zhanghao, W. Liu, et al.

Discover the groundbreaking Spectrum and Polarization Optical Tomography (SPOT) technique developed by researchers including Karl Zhanghao and Wenhui Liu, which allows for real-time monitoring of subcellular lipid dynamics in live cells. This innovative method provides insights into lipid heterogeneities during cell division across multiple organelles.

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Playback language: English
Abstract
This paper introduces a Spectrum and Polarization Optical Tomography (SPOT) technique for studying subcellular lipidomics in live cells using Nile Red staining. SPOT simultaneously resolves membrane morphology, polarity, and phase from three optical dimensions. The high-throughput optical properties reveal lipid heterogeneities across ten subcellular compartments at different developmental stages. Real-time monitoring of multi-organelle interactive activities during cell division reveals sophisticated lipid dynamics.
Publisher
Nature Communications
Published On
Nov 18, 2020
Authors
Karl Zhanghao, Wenhui Liu, Meiqi Li, Zihan Wu, Xiao Wang, Xingye Chen, Chunyan Shan, Haoqian Wang, Xiaowei Chen, Qionghai Dai, Peng Xi, Dayong Jin
Tags
Spectrum Optical Tomography
lipidomics
live cells
Nile Red staining
membrane morphology
polarization
subcellular compartments

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