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Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

Agriculture

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

L. Yang, F. Machin, et al.

This innovative research by Lei Yang, Frank Machin, Shuangfeng Wang, Eleftheria Saplaoura, and Friedrich Kragler reveals a groundbreaking method for achieving heritable, transgene-free genome edits in plants. By cleverly utilizing a fusion of Cas9 and guide RNA with tRNA-like sequences, scientists successfully transferred editing capabilities from rootstocks to shoots, paving the way for efficient plant production without the complications of transgenes.... show more
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