Heritable transgene-free genome editing in plants by grafting of | ResearchBunny

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Abstract

This research introduces a novel method for creating heritable, transgene-free genome edits in plants. By fusing Cas9 and guide RNA (gRNA) transcripts to tRNA-like sequence (TLS) motifs, the researchers enabled the movement of these editing components from transgenic rootstocks to grafted wild-type shoots (scions). This resulted in heritable gene editing in both *Arabidopsis thaliana* and *Brassica rapa*, eliminating the need for lengthy outcrossing to remove transgenes. The method offers a faster and more efficient approach to transgene-free plant production.

Publisher

Nature Biotechnology

Published On

Jul 01, 2023

Authors

Lei Yang, Frank Machin, Shuangfeng Wang, Eleftheria Saplaoura, Friedrich Kragler

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