Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells

This study details the development of a system for the directional differentiation of porcine pre-gastrulation epiblast stem cells (pgEpiSCs) into three-dimensional (3D) meat-like tissue. The researchers established a serum-free myogenic differentiation system for pgEpiSCs, demonstrating that the resulting skeletal muscle progenitor cells and fibers exhibit typical muscle cell characteristics and transcriptional features. A 3D differentiation system was created using plant-based edible scaffolds, resulting in the generation of pgEpiSCs-derived cultured meat. This approach offers a new technique for cultured meat production.

Gaoxiang Zhu, Dengfeng Gao, Linzi Li, Yixuan Yao, Yingjie Wang, Minglei Zhi, Jinying Zhang, Xinze Chen, Qianqian Zhu, Jie Gao, Tianzhi Chen, Xiaowei Zhang, Tong Wang, Suying Cao, Aijin Ma, Xianchao Feng, Jianyong Han