This study details the development of a system for the directional differentiation of porcine pre-gastrulation epiblast stem cells (pgEpiSCs) into three-dimensional (3D) meat-like tissue. The researchers established a serum-free myogenic differentiation system for pgEpiSCs, demonstrating that the resulting skeletal muscle progenitor cells and fibers exhibit typical muscle cell characteristics and transcriptional features. A 3D differentiation system was created using plant-based edible scaffolds, resulting in the generation of pgEpiSCs-derived cultured meat. This approach offers a new technique for cultured meat production.
