Biology

Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

A. Pascual-reguant, R. Köhler, et al.

This study by Anna Pascual-Reguant and colleagues unveils the intricate world of innate lymphoid cells (ILCs), exploring their localization and interactions within the tonsil microenvironment. By identifying IRF4 as a specific marker for tonsillar ILC3s, the researchers reveal shared niches with plasma cells, paving the way for deeper understanding of ILC biology through advanced histological techniques.

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Abstract

Innate lymphoid cells (ILCs) are important immune regulators, but their precise tissue localization and microenvironmental interactions remain unclear. This study combines multiplexed immunofluorescence and a computational analysis pipeline to identify CD127+ ILCs *in situ*, characterize them, and define their microenvironments. The researchers identified IRF4 as a marker for tonsillar ILC3s and conserved stromal landmarks for ILC localization, revealing shared niches with plasma cells in the tonsil. This work provides a platform for multiparametric histological analysis of ILCs to advance ILC biology understanding.

Publisher

Nature Communications

Published On

Mar 19, 2021

Authors

Anna Pascual-Reguant, Ralf Köhler, Ronja Mothes, Sandy Bauherr, Daniela C. Hernández, Ralf Uecker, Karolin Holzwarth, Katja Kotsch, Maximilian Seidl, Lars Philipsen, Werner Müller, Chiara Romagnani, Raluca Niesner, Anja E. Hauser

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