Formation of contractile 3D bovine muscle tissue for | ResearchBunny

Index

Abstract

This study presents a novel method for creating 3D cultured bovine muscle tissue with aligned myotubes that contract upon electrical stimulation. Millimeter-thick tissues were constructed by accumulating bovine myoblast-laden hydrogel modules. Optimal biomaterial composition (fibrin-matrigel mixture) and electrical stimulation (1 Hz, 2 ms duration, 3 V/mm) were determined to promote myotube maturation and contractility. The resulting tissue exhibited low microbial contamination, suggesting potential for clean cultured steak production.

Publisher

npj Science of Food

Published On

Mar 02, 2021

Authors

Mai Furuhashi, Yuya Morimoto, Ai Shima, Futoshi Nakamura, Hiroshi Ishikawa, Shoji Takeuchi

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