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Abstract
Cell-cultured meat offers a more sustainable food system. However, research is hindered by the lack of serum-free media for robust cell expansion. This study adapts a low-cost serum-free medium (B8) for bovine satellite cells by adding recombinant albumin, creating Beefy-9. Beefy-9 supports long-term satellite cell expansion (seven passages) with a 39-hour doubling time, comparable to serum-containing media. This offers a promising foundation for developing serum-free media for other species, accelerating cultured meat research.
Publisher
Communications Biology
Published On
Jun 02, 2022
Authors
Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen Jr., David L. Kaplan
Tags
cell-cultured meat
sustainable food
serum-free medium
bovine satellite cells
Beefy-9
cell expansion
tissue engineering

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