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Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

Food Science and Technology

Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

A. J. Stout, A. B. Mirliani, et al.

Explore how researchers Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen Jr., and David L. Kaplan are transforming the future of food with their innovative serum-free medium, Beefy-9, designed for long-term expansion of bovine satellite cells. This groundbreaking study promises to accelerate cultured meat research and pave the way for sustainable food systems.

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Playback language: English
Abstract
Cell-cultured meat offers a more sustainable food system. However, research is hindered by the lack of serum-free media for robust cell expansion. This study adapts a low-cost serum-free medium (B8) for bovine satellite cells by adding recombinant albumin, creating Beefy-9. Beefy-9 supports long-term satellite cell expansion (seven passages) with a 39-hour doubling time, comparable to serum-containing media. This offers a promising foundation for developing serum-free media for other species, accelerating cultured meat research.
Publisher
Communications Biology
Published On
Jun 02, 2022
Authors
Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen Jr., David L. Kaplan
Tags
cell-cultured meat
sustainable food
serum-free medium
bovine satellite cells
Beefy-9
cell expansion
tissue engineering

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