Medicine and Health

CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics

L. Hao, R. T. Zhao, et al.

This research introduces a groundbreaking method for detecting and differentiating disease states using chemically stabilized nucleic acids multiplexed with synthetic biomarkers. Conducted by renowned researchers including Liangliang Hao and Sangeeta N. Bhatia, this innovative approach utilizes urine analysis through CRISPR-Cas detection mechanisms, paving the way for a convenient point-of-care diagnostic tool.

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Abstract

This research introduces chemically stabilized nucleic acids to multiplex synthetic biomarkers, enabling the detection and differentiation of disease states in murine cancer models through non-invasive urine analysis. The method employs microenvironmental endopeptidase to trigger barcode release and CRISPR-Cas-mediated detection, culminating in a point-of-care paper diagnostic tool and a microfluidic platform for high-throughput analysis.

Publisher

Nature Nanotechnology

Published On

Apr 24, 2023

Authors

Liangliang Hao, Renee T. Zhao, Nicole L. Welch, Edward Kah Wei Tan, Qian Zhong, Nour Saida Harzallah, Chayanon Ngambenjawong, Henry Ko, Heather E. Fleming, Pardis C. Sabeti, Sangeeta N. Bhatia

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