This research investigated the chondrogenic differentiation of human Wharton's Jelly mesenchymal stem cells (hWJ-MSCs) on silk scaffolds. Silk scaffolds were fabricated by mixing silk fibroin (SF) from *Bombyx mori* with silk spidroin (SS) from *Argiope appensa*, using a salt leaching method. Chondrogenesis was induced using L-ascorbic acid (LAA) or platelet-rich plasma (PRP). The optimal scaffold composition (10% SS + 90% SF) enhanced cell proliferation and, when supplemented with 10% PRP, supported the best chondrogenesis, as evidenced by Alcian Blue staining and collagen type II immunocytochemistry. The addition of spidroin improved scaffold compressive strength and cell attachment, possibly due to the presence of RGD sequences.
