Medicine and Health

Simulated lunar microgravity transiently arrests growth and induces osteocyte-chondrocyte lineage differentiation in human Wharton's jelly stem cells

A. Subramanian, C. H. L. Ip, et al.

This groundbreaking research explores the impact of simulated lunar microgravity on human Wharton's jelly stem cells (hWJSCs). Conducted by an expert team of researchers, the study uncovers how exposure to microgravity induces reversible changes in cell growth and differentiation towards osteocyte-chondrocyte lineages, providing exciting insights for future biotechnological applications.

00:00

Playback language: English

Index

Abstract

Human Wharton's jelly stem cells (hWJSCs) are multipotent stem cells used in biotechnology. This study investigated the effects of simulated lunar microgravity (sµG) on hWJSC growth, differentiation, and viability. 72-hour sµG exposure significantly reduced hWJSC growth but not viability, decreasing expression of stemness markers (CD73, CD90, CD105) and key replication markers like MKI67. Conversely, osteocyte-chondrocyte lineage markers (SERPINI1, MSX2, etc.) were upregulated. Upon return to 1.0 G, growth accelerated, and stemness marker expression recovered. The findings suggest that acute sµG induces reversible growth changes and osteocyte-chondrocyte lineage differentiation in hWJSCs.

Publisher

npj Microgravity

Published On

Mar 01, 2024

Authors

Arjunan Subramanian, Chelsea Han Lin IP, Wei Qin, Xiawen Liu, Sean W.D. Carter, Gokce Oguz, Adaikalavan Ramasamy, Sebastian E. Illanes, Arijit Biswas, Gabriel G. Perron, Erin L. Fee, Sarah W. L. Li, Michelle K.Y. Seah, Mahesh A. Choolani, Matthew W. Kemp

Related Publications

Explore these studies to deepen your understanding of the subject.

Engineering and Technology

Chondrogenic differentiation of Wharton's Jelly mesenchymal stem cells on silk spidroin-fibroin mix scaffold supplemented with L-ascorbic acid and platelet rich plasma

A. Barlian, H. Judawisastra, et al.

Medicine and Health

Human cytomegalovirus in breast milk is associated with milk composition and the infant gut microbiome and growth

K. E. Johnson, N. Hernandez-alvarado, et al.

Medicine and Health

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells

L. Cheng, M. Xie, et al.

Medicine and Health

Differential routing and disposition of the long-chain saturated fatty acid palmitate in rodent vs human beta-cells

P. Thomas, C. Arden, et al.

Listen, Learn & Level Up

Over 10,000 hours of research content in 25+ fields, available in 12+ languages.

No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.

listen to research audio papers with researchbunny