Medicine and Health

Simulated lunar microgravity transiently arrests growth and induces osteocyte-chondrocyte lineage differentiation in human Wharton's jelly stem cells

A. Subramanian, C. H. L. Ip, et al.

This groundbreaking research explores the impact of simulated lunar microgravity on human Wharton's jelly stem cells (hWJSCs). Conducted by an expert team of researchers, the study uncovers how exposure to microgravity induces reversible changes in cell growth and differentiation towards osteocyte-chondrocyte lineages, providing exciting insights for future biotechnological applications.

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Abstract

Human Wharton's jelly stem cells (hWJSCs) are multipotent stem cells used in biotechnology. This study investigated the effects of simulated lunar microgravity (sµG) on hWJSC growth, differentiation, and viability. 72-hour sµG exposure significantly reduced hWJSC growth but not viability, decreasing expression of stemness markers (CD73, CD90, CD105) and key replication markers like MKI67. Conversely, osteocyte-chondrocyte lineage markers (SERPINI1, MSX2, etc.) were upregulated. Upon return to 1.0 G, growth accelerated, and stemness marker expression recovered. The findings suggest that acute sµG induces reversible growth changes and osteocyte-chondrocyte lineage differentiation in hWJSCs.

Publisher

npj Microgravity

Published On

Mar 01, 2024

Authors

Arjunan Subramanian, Chelsea Han Lin IP, Wei Qin, Xiawen Liu, Sean W.D. Carter, Gokce Oguz, Adaikalavan Ramasamy, Sebastian E. Illanes, Arijit Biswas, Gabriel G. Perron, Erin L. Fee, Sarah W. L. Li, Michelle K.Y. Seah, Mahesh A. Choolani, Matthew W. Kemp

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