This research paper analyzes the N-glycosylation profiles of recombinant spike proteins from four SARS-CoV-2 variants of concern (Alpha, Beta, Gamma, and Delta), along with the D614G variant as a control. Mass spectrometric analysis reveals that amino acid substitutions and deletions impact the abundance and type of glycans on spike protein glycosylation sites. While showing high similarity to the D614G variant in site-specific glycan content, some glycosylation sequons exhibit differences in glycan form distribution between variants. Multiple digestion methods were used to improve glycopeptide identification and quantification. The detailed analysis provides insights into the role of protein glycosylation in viral protein structure, function, and the development of vaccines and therapeutics.
Publisher
Analytical and Bioanalytical Chemistry
Published On
Jun 24, 2023
Authors
Dongxia Wang, Jakub Baudys, Sarah H Osman, John R Barr
Tags
N-glycosylation
SARS-CoV-2
spike proteins
variant analysis
glycopeptide identification
vaccine development
therapeutics
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