Biology

Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2

D. Wang, J. Baudys, et al.

This exciting research conducted by Dongxia Wang, Jakub Baudys, Sarah H Osman, and John R Barr delves into the N-glycosylation profiles of SARS-CoV-2 spike proteins across multiple variants. Discover how specific amino acid changes influence glycan types and distribution, shedding light on the implications for vaccine and therapeutic development.

00:00

Playback language: English

Index

Abstract

This research paper analyzes the N-glycosylation profiles of recombinant spike proteins from four SARS-CoV-2 variants of concern (Alpha, Beta, Gamma, and Delta), along with the D614G variant as a control. Mass spectrometric analysis reveals that amino acid substitutions and deletions impact the abundance and type of glycans on spike protein glycosylation sites. While showing high similarity to the D614G variant in site-specific glycan content, some glycosylation sequons exhibit differences in glycan form distribution between variants. Multiple digestion methods were used to improve glycopeptide identification and quantification. The detailed analysis provides insights into the role of protein glycosylation in viral protein structure, function, and the development of vaccines and therapeutics.

Publisher

Analytical and Bioanalytical Chemistry

Published On

Jun 24, 2023

Authors

Dongxia Wang, Jakub Baudys, Sarah H Osman, John R Barr

Related Publications

Explore these studies to deepen your understanding of the subject.

Medicine and Health

The COVID-AGICT study: COVID-19 and advanced gastro-intestinal cancer surgical treatment. A multicentric Italian study on the SARS-CoV-2 pandemic impact on gastro-intestinal cancers surgical treatment during the 2020. Analysis of perioperative and short-term oncological outcomes

G. Giuliani, F. Guerra, et al.

Political Science

Media events, speech events and propagandistic techniques of legitimation: a multimodal analysis of the Greek Prime Minister Kyriakos Mitsotakis' public addresses on the SARS-CoV-2 pandemic

S. Poulakidakos

Medicine and Health

Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells

W. Hong, J. Yang, et al.

Medicine and Health

Risk factors for and pregnancy outcomes after SARS-CoV-2 in pregnancy according to disease severity: A nationwide cohort study with validation of the SARS-CoV-2 diagnosis of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG)

A. J. M. Aabakke, T. G. Petersen, et al.

Listen, Learn & Level Up

Over 10,000 hours of research content in 25+ fields, available in 12+ languages.

No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.

listen to research audio papers with researchbunny