Biology

A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation

M. Scheffen, D. G. Marchal, et al.

Discover the groundbreaking development of glycolyl-CoA carboxylase (GCC), an innovative enzyme designed to significantly enhance CO2 fixation. This research by Marieke Scheffen and colleagues demonstrates GCC's remarkable catalytic efficiency improvement and its potential to increase carbon efficiency by up to 150%, revolutionizing sustainable processes.

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Index

Abstract

This paper presents the development of glycolyl-CoA carboxylase (GCC), a novel enzyme engineered to enhance CO2 fixation. Through rational design, high-throughput microfluidics, and microplate screens, GCC's catalytic efficiency was improved by three orders of magnitude. The redesigned active site was validated using cryo-electron microscopy. A carboxylation module, including GCC, was created for converting glycolate (C2) to glycerate (C3), and its application in photorespiration, ethylene glycol conversion, and synthetic CO2 fixation was demonstrated. Stoichiometric calculations suggest GCC could increase carbon efficiency by up to 150% and reduce energy demand.

Publisher

Nature Catalysis

Published On

Jan 04, 2021

Authors

Marieke Scheffen, Daniel G. Marchal, Thomas Beneyton, Sandra K. Schuller, Melanie Klose, Christoph Diehl, Jessica Lehmann, Pascal Pfister, Martina Carrillo, Hai He, Selçuk Aslan, Niña S. Cortina, Peter Claus, Daniel Bollschweiler, Jean-Christophe Baret, Jan M. Schuller, Jan Zarzycki, Arren Bar-Even, Tobias J. Erb

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