Medicine and Health

A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples

Y. Tang, T. Song, et al.

This groundbreaking research led by Yanan Tang and colleagues introduces an ultrasensitive CRISPR-based antibody detection assay for anti-SARS-CoV-2 antibodies, proving 10,000 times more sensitive than traditional methods. Its remarkable 100% sensitivity and 98.5% specificity may revolutionize antibody detection, particularly for immunocompromised individuals.

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Abstract

This paper introduces an ultrasensitive CRISPR-based antibody detection (UCAD) assay for detecting anti-SARS-CoV-2 antibodies. UCAD is significantly more sensitive than traditional immunoassays (10,000 times more sensitive than ELISA), exhibiting 100% sensitivity and 98.5% specificity in clinical validation. Its high sensitivity allows for the detection of antibodies in immunocompromised individuals where standard assays fail. The assay's simplicity and sensitivity suggest potential for widespread clinical application in various settings.

Publisher

Nature Communications

Published On

Aug 09, 2022

Authors

Yanan Tang, Turun Song, Lu Gao, Saifu Yin, Ming Ma, Yun Tan, Lijuan Wu, Yang Yang, Yanquan Wang, Tao Lin, Feng Li

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