Medicine and Health

3D-printed TCP-HA scaffolds delivering MicroRNA-302a-3p improve bone regeneration in a mouse calvarial model

P. Limlawan, N. Insin, et al.

This study by Pirawish Limlawan and colleagues explores the remarkable potential of hydroxyapatite nanoparticles modified with 3-aminopropyltriethoxysilane to enhance bone regeneration in critical-sized calvarial defects in mice. The innovative approach significantly improves microRNA delivery and promotes earlier new bone formation, paving the way for future advancements in bone healing therapies.

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Abstract

This study investigated whether hydroxyapatite nanoparticles modified with 3-aminopropyltriethoxysilane (HA-NPs-APTES) and carrying microRNA-302a-3p (miR) within a 3D-printed tricalcium phosphate/hydroxyapatite (TCP/HA) scaffold could enhance bone regeneration in a critical-sized mouse calvarial defect. Two modification methods (M1, M2) were compared. M2, involving surface application of HA-NPs-APTES-miR, showed superior miR delivery, downregulating COUP-TFII and upregulating RUNX2 mRNA. In vivo, M2 scaffolds significantly increased BV/TV and reduced unfilled spaces compared to controls. Histomorphometry showed earlier new bone formation in the M2 group. The modified TCP/HA scaffold facilitated miR delivery and enhanced bone regeneration.

Publisher

BDJ Open

Published On

Nov 24, 2023

Authors

Pirawish Limlawan, Numpon Insin, Laurine Marger, Mélanie Freudenreich, Stéphane Durual, Anjalee Vacharaksa

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