Medicine and Health

Streamlined and on-demand preparation of mRNA products on a universal integrated platform

H. Wei, Z. Rong, et al.

Discover an innovative platform that streamlines the preparation of mRNA products from DNA templates, achieving impressive production rates of eGFP mRNA. Developed by a dedicated team of researchers led by Hongjuan Wei and colleagues, this technology paves the way for rapid development of mRNA-based vaccines and therapies against emerging diseases.

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Abstract

This study presents a universal integrated platform for the streamlined and on-demand preparation of mRNA products directly from DNA templates. The platform incorporates PCR amplification of target DNA, in vitro transcription (IVT) into mRNA, magnetic purification, and encapsulation in lipid nanoparticles (LNPs). Using enhanced green fluorescent protein (eGFP) as a model, the platform achieved a production rate of 200–300 µg of eGFP mRNA in 8 h. Furthermore, an mRNA vaccine encoding the receptor-binding domain (RBD) of SARS-CoV-2 spike protein was successfully produced. This platform offers advantages for universal and on-demand mRNA product preparation, facilitating broader access to mRNA technology and enabling rapid development of mRNA-based vaccines and therapies.

Publisher

Microsystems & Nanoengineering

Published On

Jan 28, 2023

Authors

Hongjuan Wei, Zhen Rong, Liyan Liu, Ye Sang, Jing Yang, Shengqi Wang

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