Biology

Single-particle cryo-EM structures from IDPC-STEM at near-atomic resolution

I. Lazić, M. Wirix, et al.

This groundbreaking study showcases the potential of integrated differential phase contrast scanning transmission electron microscopy (IDPC-STEM) for high-resolution structure determination in cryo-electron microscopy. By examining keyhole limpet hemocyanin and tobacco mosaic virus, the researchers achieved near-atomic resolution, establishing IDPC-STEM as a powerful tool for biological macromolecule analysis. This research was conducted by Ivan Lazić, Maarten Wirix, Max Leo Leidl, Felix de Haas, Daniel Mann, Maximilian Beckers, Evgeniya V. Pechnikova, Knut Müller-Caspary, Ricardo Egoavil, Eric G. T. Bosch, and Carsten Sachse.

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Abstract

This study explores the application of integrated differential phase contrast scanning transmission electron microscopy (IDPC-STEM) for cryo-electron microscopy (cryo-EM) structure determination. Using keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV) as test specimens, the authors demonstrate that IDPC-STEM can produce high-resolution images suitable for single-particle reconstruction. Near-atomic resolution structures were obtained for KLH (6.5 Å) and TMV (3.5 Å), matching results from conventional transmission electron microscopy (CTEM). This work establishes IDPC-STEM as a viable technique for high-resolution cryo-EM structure determination of biological macromolecules.

Publisher

Nature Methods

Published On

Sep 05, 2022

Authors

Ivan Lazić, Maarten Wirix, Max Leo Leidl, Felix de Haas, Daniel Mann, Maximilian Beckers, Evgeniya V. Pechnikova, Knut Müller-Caspary, Ricardo Egoavil, Eric G. T. Bosch, Carsten Sachse

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