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Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids

Biology

Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids

J. Wang, N. Xue, et al.

Discover GECFINDER, a groundbreaking method developed by Jin Wang and colleagues that converts enzymes into vibrant biosensors in record time. Experience how ligand-induced conformational changes lead to quantifiable fluorescence signals for various organic and amino acids, simplifying amino acid quantification for practical applications. This innovative approach promises to revolutionize point-of-care testing and high-throughput screening in industry.

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Playback language: English
Abstract
Rapid construction of diverse genetically encoded biosensors remains challenging. This paper presents GECFINDER, a method to rapidly convert enzymes into biosensors. By inserting a circularly permuted fluorescent protein (cpFP) into the hinges of ANL superfamily enzymes, ligand-induced conformational changes are translated into fluorescence signal changes. The authors generated 11 GECFINDERS for various organic and amino acids, demonstrating their efficacy in quantifying target molecules in vitro. GECFINDER simplifies amino acid quantification, showing promise for point-of-care testing and high-throughput screening in industrial applications.
Publisher
Nature Communications
Published On
Oct 21, 2023
Authors
Jin Wang, Ning Xue, Wenjia Pan, Ran Tu, Shixin Li, Yue Zhang, Yufeng Mao, Ye Liu, Haijiao Cheng, Yanmei Guo, Wei Yuan, Xiaomeng Ni, Meng Wang
Tags
biosensors
enzyme
fluorescence
amino acids
GECFINDER
quantification
high-throughput

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