Rapid discovery of self-assembling peptides with one-bead one-compound peptide library

This paper presents a novel method for rapidly identifying self-assembling peptides using a one-bead one-compound (OBOC) combinatorial library. The method utilizes a hydrophobicity-sensitive fluorescent molecule, NBD, attached to the N-terminus of peptides. Self-assembling peptides create hydrophobic pockets, activating NBD fluorescence. This approach successfully identified eight pentapeptides that self-assemble into nanoparticles or nanofibers, some of which interact with and are taken up by HeLa cells. The method is efficient and simple, offering a rapid means of discovering self-assembling peptides for biomedical and material applications.

Pei-Pei Yang, Yi-Jing Li, Yan Cao, Lu Zhang, Jia-Qi Wang, Ziwei Lai, Kuo Zhang, Diedra Shorty, Wenwu Xiao, Hui Cao, Lei Wang, Hao Wang, Ruiwu Liu, Kit S. Lam