Biology

NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals

N. Mougios, E. R. Cotroneo, et al.

Experience a breakthrough in fluorescence microscopy with NanoPlex, a method developed by Nikolaos Mougios and colleagues. This innovative approach utilizes engineered nanobodies for selective signal removal, enabling unprecedented analysis of multiple targets in 3D confocal and super-resolution imaging.

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Abstract

This paper introduces NanoPlex, a streamlined method for fluorescence microscopy multiplexing using engineered secondary nanobodies that allow the selective removal of fluorescence signals. Three complementary signal removal strategies are developed: OptoPlex (light-induced), EnzyPlex (enzymatic), and ChemiPlex (chemical). NanoPlex achieves 21 targets for 3D confocal analysis and 5–8 targets for super-resolution imaging (dSTORM and STED), offering a potential revolution in multi-target fluorescent imaging.

Publisher

Nature Communications

Published On

Oct 10, 2024

Authors

Nikolaos Mougios, Elena R. Cotroneo, Nils Imse, Jonas Setzke, Silvio O. Rizzoli, Nadja A. Simeth, Roman Tsukanov, Felipe Opazo

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