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Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging

Biology

Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging

M. Strotton, T. Hosogane, et al.

Discover the groundbreaking multielement Z-tag X-ray fluorescence (MEZ-XRF) bioimaging method developed by Merrick Strotton, Tsuyoshi Hosogane, Marco di Michiel, Holger Moch, Zsuzsanna Varga, and Bernd Bodenmiller. This innovative technique allows for rapid, high-resolution imaging of subcellular features, marking a significant advancement in multiomic tissue analysis.

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Playback language: English
Abstract
This paper introduces multielement Z-tag X-ray fluorescence (MEZ-XRF), a novel high-speed, highly multiplexed bioimaging method. MEZ-XRF uses X-ray-sensitive reporter tags (Z-tags) based on different elements to achieve fluorescence-type imaging. Combined with signal amplification by exchange reaction (SABER), MEZ-XRF enables parallel imaging of numerous markers at subcellular resolution in various samples. The method is demonstrated to be nondestructive and capable of multiscale imaging, allowing for rapid tissue overviews followed by high-resolution scans of specific regions of interest. MEZ-XRF's unique capabilities are benchmarked against imaging mass cytometry (IMC) and showcase its potential for advanced multiomic tissue analyses.
Publisher
Nature Methods
Published On
Sep 01, 2023
Authors
Merrick Strotton, Tsuyoshi Hosogane, Marco di Michiel, Holger Moch, Zsuzsanna Varga, Bernd Bodenmiller
Tags
bioimaging
X-ray fluorescence
MEZ-XRF
multiomic
signal amplification
subcellular resolution
tissue analysis
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