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Magnify is a universal molecular anchoring strategy for expansion microscopy

Biology

Magnify is a universal molecular anchoring strategy for expansion microscopy

A. Klimas, B. R. Gallagher, et al.

Discover how Magnify revolutionizes expansion microscopy by enabling the retention of biomolecules without separate anchoring steps. This innovative method provides remarkable 11x expansion and 25 nm resolution, revealing stunning nanoscale subcellular structures. Conducted by a team of experts from Carnegie Mellon University and Brown University.

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Playback language: English
Abstract
Expansion microscopy (ExM) physically magnifies biological specimens for nanoscale imaging. Current ExM protocols require anchoring steps to link biomolecules to the hydrogel. This paper introduces Magnify, a method using a robust hydrogel that retains nucleic acids, proteins, and lipids without separate anchoring, achieving up to 11x expansion and 25 nm effective resolution on conventional microscopes. Magnify is demonstrated on various specimens, revealing nanoscale subcellular structures.
Publisher
Nature Biotechnology
Published On
Jun 01, 2023
Authors
Aleksandra Klimas, Brendan R. Gallagher, Piyumi Wijesekara, Sinda Fekir, Emma F. DiBernardo, Zhangyu Cheng, Donna B. Stolz, Franca Cambi, Simon C. Watkins, Steven L. Brody, Amjad Horani, Alison L. Barth, Christopher I. Moore, Xi Ren, Yongxin Zhao
Tags
Expansion Microscopy
Nanoscale Imaging
Hydrogel
Biomolecules
Subcellular Structures
Effective Resolution
Magnify

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