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Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Biology

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

V. Zickus, M. Wu, et al.

This groundbreaking research on scan-less wide-field FLIM reveals a significant leap in cellular imaging, achieving an impressive acquisition rate of 1 Hz using a novel time-gated SPAD camera. With an astonishing 1000-fold acceleration in fluorescence lifetime estimation through a pre-trained neural network, this study promises transformative potential for real-time applications, conducted by Vytautas Zickus and colleagues.

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