Veterinary Science

Evaluation of enzymatic protocols to optimize efficiency of bovine adipose tissue-derived mesenchymal stromal cell isolation

E. Heyman, B. Devriendt, et al.

This groundbreaking study by Emma Heyman and colleagues explores innovative isolation techniques for bovine mesenchymal stromal cells from adipose tissue. The team identified 0.1% Liberase™ for 3 hours as the optimal condition, significantly enhancing cell yield and myogenic differentiation, pivotal for cultured meat production.

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Playback language: English

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Abstract

This study evaluates 32 isolation conditions using four enzyme mixtures (Collagenase type I, Collagenase type I + Trypsin, Liberase™, and Collagenase type IV) at varying concentrations and incubation times to isolate bovine mesenchymal stromal cells (MSCs) from adipose tissue. Liberase™ at 0.1% for 3 hours yielded the highest cell count with a low population doubling time. MSC identity was confirmed, and myogenic differentiation was achieved using 5-aza-2'-deoxycytidine and galectin-1, increasing myogenic regulatory factor mRNA levels. For cultured meat production using bovine AT-MSCs, 0.1% Liberase™ for 3 hours is recommended.

Publisher

npj Science of Food

Published On

Oct 01, 2024

Authors

Emma Heyman, Bert Devriendt, Elly De Vlieghere, Klara Goethals, Mario Van Poucke, Luc Peelman, Catharina De Schauwer

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