Biology

Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system

Z. Zhong, G. Liu, et al.

Discover an innovative plant genome engineering system utilizing CRISPR-Cas9 from the probiotic *Lactobacillus rhamnosus*, which enhances editing efficiency in diverse plant species. This groundbreaking research was conducted by Zhaohui Zhong, Guanqing Liu, Zhongjie Tang, Shuyue Xiang, Liang Yang, Lan Huang, Yao He, Tingting Fan, Shishi Liu, Xuelian Zheng, Tao Zhang, Yiping Qi, Jian Huang, and Yong Zhang.

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Abstract

This study introduces an efficient plant genome engineering system using CRISPR-Cas9 from the probiotic *Lactobacillus rhamnosus*, surpassing existing systems in editing efficiency across various plant species. LrCas9, the *L. rhamnosus* Cas9, demonstrates high specificity and facilitates multiplexed gene knockout and knockdown. The development of LrCas9-derived base editors and CRISPR interference/activation systems further expands its applications in plant genome engineering.

Publisher

Nature Communications

Published On

Sep 29, 2023

Authors

Zhaohui Zhong, Guanqing Liu, Zhongjie Tang, Shuyue Xiang, Liang Yang, Lan Huang, Yao He, Tingting Fan, Shishi Liu, Xuelian Zheng, Tao Zhang, Yiping Qi, Jian Huang, Yong Zhang

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