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Transposase-assisted target-site integration for efficient plant genome engineering

Biology

Transposase-assisted target-site integration for efficient plant genome engineering

P. Liu, K. Panda, et al.

Discover a groundbreaking genome engineering tool that harnesses the power of transposable elements to safely and accurately insert DNA into plant genomes. Led by researchers including Peng Liu and Kaushik Panda from the Donald Danforth Plant Science Center, this innovative system offers a leap forward in agricultural biotechnology.

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Playback language: English
Abstract
Current plant genome engineering technologies for inserting new DNA into specific locations are inefficient and error-prone. This study developed a genome engineering tool that leverages the precise insertion capabilities of transposable elements (TEs). By fusing the rice Pong transposase protein to Cas9 or Cas12a nucleases, the researchers achieved sequence-specific targeted insertion of various DNA sequences, including enhancer elements and gene expression cassettes, into the genomes of Arabidopsis and soybean. This transposase-assisted targeted insertion system (TATSI) demonstrated higher efficiency and accuracy compared to existing methods.
Publisher
Nature
Published On
Jul 18, 2024
Authors
Peng Liu, Kaushik Panda, Seth A. Edwards, Ryan Swanson, Hochul Yi, Pratheek Pandesha, Yu-Hung Hung, Gerald Klaas, Xudong Ye, Megan V. Collins, Kaili N. Renken, Larry A. Gilbertson, Veena Veena, C. Nathan Hancock, R. Keith Slotkin
Tags
genome engineering
transposable elements
DNA insertion
agricultural biotechnology
Arabidopsis
soybean
TATSI

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