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Abstract
Cultivated meat production needs bioprocess optimization to achieve high cell densities. Edible microcarriers, such as inactivated mycelium from edible fungi, offer a solution by providing a large surface area for cell adhesion and proliferation, while also integrating into the final product. This study investigated the impact of different fungal species on C2C12 myoblast and bovine satellite cell (bSC) growth and differentiation on mycelium carriers. *Aspergillus oryzae* proved most effective for cell proliferation. Both C2C12 and bSC cells differentiated on mycelium carriers, demonstrating the potential of this technology for cultivated meat production.
Publisher
npj Science of Food
Published On
Apr 30, 2024
Authors
Minami Ogawa, Alex S. Kermani, Mayrene J. Huynh, Keith Baar, J. Kent Leach, David E. Block
Tags
cultivated meat
bioprocess optimization
mycelium carriers
cell proliferation
differentiation
fungal species
C2C12 myoblast

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