This paper explores the clinical potential of E-DNA scaffold sensors for multiplexed, point-of-care antibody measurement. The sensors utilize a rigid DNA scaffold attached to an electrode, with a redox reporter whose electron transfer is hindered by antibody binding. The researchers developed E-DNA sensors targeting three HIV-specific antibodies and compared their performance to ELISA and lateral flow immunoassays using human serum samples. While ELISA showed lower detection limits due to amplification, the clinical sensitivity of all three methods was comparable. The E-DNA sensors offer a potential improvement by combining the quantification and multiplexing of ELISA with the speed and convenience of point-of-care tests.

Claudio Parolo, Ava S. Greenwood, Nathan E. Ogden, Di Kang, Chase Hawes, Gabriel Ortega, Netzahualcóyotl Arroyo-Currás, Kevin W. Plaxco