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Design of a recombinant asparaginyl ligase for site-specific modification using efficient recognition and nucleophile motifs

Biology

Design of a recombinant asparaginyl ligase for site-specific modification using efficient recognition and nucleophile motifs

J. Tang, M. Hao, et al.

This groundbreaking research by Jiabao Tang, Mengling Hao, Junxian Liu, Yaling Chen, Gulimire Wufuer, Jie Zhu, Xuejie Zhang, Tingquan Zheng, Mujin Fang, Shiyin Zhang, Tingdong Li, Shengxiang Ge, Jun Zhang, and Ningshao Xia introduces a high-performance asparaginyl ligase, OaAEP1-C247A-aa55-351, capable of efficient bioconjugation. With remarkable 70-fold enhanced activity, this innovation is set to revolutionize protein engineering and drug development across various pH levels.

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