Chemistry

A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy

R. Lincoln, M. L. Bossi, et al.

This groundbreaking research introduces PaX dyes, innovative caging-group-free photoactivatable fluorophores that transition into highly fluorescent pyronine dyes upon light activation. Conducted by Richard Lincoln, Mariano L. Bossi, Michael Remmel, Elisa D’Este, Alexey N. Butkevich, and Stefan W. Hell, this method offers a versatile and efficient approach to microscopy, enabling detailed imaging for both fixed and live cells across various techniques.

00:00

Playback language: English

Index

Abstract

This paper introduces a novel method for creating caging-group-free photoactivatable fluorophores (PaX dyes) based on 3,6-diaminoxanthones. These dyes efficiently and cleanly convert into highly fluorescent pyronine dyes upon light irradiation, offering advantages over traditional methods that rely on photolabile protecting groups. The PaX strategy is versatile, applicable to various xanthone analogues, and produces fluorophores spanning the visible spectrum. The authors demonstrate PaX dyes' utility in various microscopy techniques (conventional, STED, PALM, MINFLUX) for both fixed and live-cell imaging.

Publisher

Nature Chemistry

Published On

Jul 21, 2022

Authors

Richard Lincoln, Mariano L. Bossi, Michael Remmel, Elisa D’Este, Alexey N. Butkevich, Stefan W. Hell

Related Publications

Explore these studies to deepen your understanding of the subject.

Engineering and Technology

A framework for the general design and computation of hybrid neural networks

R. Zhao, Z. Yang, et al.

Chemistry

A generative artificial intelligence framework based on a molecular diffusion model for the design of metal-organic frameworks for carbon capture

H. Park, X. Yan, et al.

Psychology

Physical Activity for Cognitive Health: A Model for Intervention Design for People Experiencing Cognitive Concerns and Symptoms of Depression or Anxiety

B. Stubbs, D. Vancampfort, et al.

Health and Fitness

Reliability and validity of a General Nutrition Knowledge Questionnaire for adults in a Romanian population

S. Putnoky, A. M. Banu, et al.

Listen, Learn & Level Up

Over 10,000 hours of research content in 25+ fields, available in 12+ languages.

No more digging through PDFs, just hit play and absorb the world's latest research in your language, on your time.

listen to research audio papers with researchbunny