This study explores the use of lysed and dried bacterial biomass from *Rhodovulum sulfidophilum* as a sustainable nitrogen fertilizer. The biomass, containing approximately 11% nitrogen, showed no negative effects on plant germination and growth at application rates up to four times that of a conventional mineral fertilizer. While requiring roughly twice the application rate of the mineral fertilizer for comparable plant growth, this is attributed to the 62% mineralization rate of bacterial nitrogen. The research demonstrates the potential of *R. sulfidophilum* as a viable nitrogen fertilizer source.