Medicine and Health

Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium

A. L. Pinto, R. K. Rai, et al.

Explore groundbreaking research that delves into the infection of human airway epithelium by SARS-CoV-2 variants, including the B.1.1.7 strain. This study, conducted by a team from Royal Brompton Hospital and Imperial College London, reveals fascinating insights into viral entry mechanisms and intracellular behavior.

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Abstract

This study used transmission electron microscopy and tomography to examine human airway epithelium infected with three SARS-CoV-2 isolates, including the B.1.1.7 variant. The virus infected ciliated but not goblet cells. Entry molecules ACE2 and TMPRSS2 were localized to the plasma membrane and microvilli but not cilia. Viral fusion at the plasma membrane was observed, releasing nucleocapsids. Intracellular virions were found in single-membrane compartments, with tomography suggesting budding into these compartments, explaining envelope acquisition. No differences in infection characteristics were observed between isolates.

Publisher

Nature Communications

Published On

Mar 25, 2022

Authors

Andreia L. Pinto, Ranjit K. Rai, Jonathan C. Brown, Paul Griffin, James R. Edgar, Anand Shah, Aran Singanayagam, Claire Hogg, Wendy S. Barclay, Clare E. Futter, Thomas Burgoyne

DOI

https://doi.org/10.1038/s41467-022-29255-y

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