This paper presents SCOPE, a single-step CRISPR-based diagnostic platform for rapid and sensitive detection of monkeypox virus (MPXV). SCOPE uses a streamlined viral lysis protocol and a 10-minute single-step recombinase polymerase amplification (RPA)-CRISPR/Cas13 reaction, conducted within a portable device. The platform can detect as few as 0.5 copies/µL of MPXV within 15 minutes and showed 100% concordance with real-time PCR in a clinical sample validation study (n=102).
Publisher
Nature Communications
Published On
Apr 16, 2024
Authors
Yunxiang Wang, Hong Chen, Kai Lin, Yongjun Han, Zhixia Gu, Hongjuan Wei, Kai Mu, Dongfeng Wang, Liyan Liu, Ronghua Jin, Rui Song, Zhen Rong, Shengqi Wang
