While axolotls can fully regenerate amputated limbs, their bone fracture healing mechanism is unclear. This study presents a surgical technique to stabilize axolotl femur osteotomies using a fixator plate, comparing it to non-stabilized osteotomy and limb amputation. Plate-fixated femurs healed more efficiently. Similar to mice, PCNA+ proliferating cells and SOX9-expressing cells were observed in early stages, suggesting endochondral ossification as the healing mechanism. This standardized fixation technique enables controlled axolotl bone healing experiments comparable to mammals.
Publisher
npj Regenerative Medicine
Published On
Jun 30, 2022
Authors
A. Polikarpova, A. Ellinghaus, O. Schmidt-Bleek, L. Grosser, C. H. Bucher, G. N. Duda, E. M. Tanaka, K. Schmidt-Bleek
Tags
axolotls
limb regeneration
osteotomy
bone healing
surgical technique
endochondral ossification
PCNA
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