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Abstract
This study introduces a handheld 3D printer for fabricating porous, cell-laden methacrylated gelatin (GelMa) constructs. The printer uses air injection and a bubble-making system with mesh filters to create high porosity (≈97%). The pore size and foamability are controlled by adjusting rheological properties of GelMa, filter size and number, and air-bioink ratio. In vitro and in vivo tests using human adipose stem cells (hASCs) showed good cell viability and significant muscle regeneration in a mouse model, suggesting this method's potential for muscle tissue regeneration.
Publisher
npj Regenerative Medicine
Published On
May 17, 2023
Authors
SeoYul Jo, JiUn Lee, Hyeongjin Lee, Dongryeol Ryu, GeunHyung Kim
Tags
3D printing
porous constructs
methacrylated gelatin
tissue regeneration
cell viability
human adipose stem cells
muscle tissue

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