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Spent media analysis suggests cultivated meat media will require species and cell type optimization

Food Science and Technology

Spent media analysis suggests cultivated meat media will require species and cell type optimization

E. N. O'neill, J. C. Ansel, et al.

This groundbreaking study reveals how chicken muscle precursor cells, chicken fibroblasts, and murine C2C12 myoblasts utilize nutrients in significantly different ways, challenging the idea of a universal culture medium for cultivated meat. Conducted by Edward N. O'Neill, Joshua C. Ansel, Grace A. Kwong, Michael E. Plastino, Jenny Nelson, Keith Baar, and David E. Block, this research emphasizes the necessity for tailored media optimization strategies.

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Playback language: English
Abstract
Cell culture media design is a major hurdle in cultivated meat commercialization. This study uses spent medium analysis (SMA) to compare nutrient utilization of chicken muscle precursor cells, chicken fibroblasts, and murine C2C12 myoblasts. Results show significant differences in nutrient utilization patterns, with glucose consumption varying considerably. The findings suggest that a single, cost-effective medium for all cell types is unlikely, emphasizing the need for streamlined media optimization methods.
Publisher
npj Science of Food
Published On
Sep 29, 2022
Authors
Edward N. O'Neill, Joshua C. Ansel, Grace A. Kwong, Michael E. Plastino, Jenny Nelson, Keith Baar, David E. Block
Tags
cultivated meat
cell culture
nutrient utilization
spent medium analysis
media optimization
chicken muscle precursor cells
C2C12 myoblasts

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