Spatially resolved multiomics on the neuronal effects induced by spaceflight in mice

This study investigated the impact of spaceflight on the mouse brain using single-cell multiomics (transcriptomics and chromatin accessibility) integrated with spatial transcriptomics. Analysis revealed significant alterations in neurogenesis, synaptogenesis, synaptic transmission, astrocyte activation, and immune dysfunction, particularly in the cortex, hippocampus, striatum, and neuroendocrine structures. Spaceflight-induced changes showed similarities to neurodegenerative disorders, characterized by oxidative stress and protein misfolding. The integrated approach provides insights into spaceflight-induced CNS impairments at the level of individual brain regions and cell types.

Yuvarani Masarapu, Egle Cekanaviciute, Zaneta Andrusivova, Jakub O. Westholm, Åsa Björklund, Robin Fallegger, Pau Badia-i-Mompel, Valery Boyko, Shubha Vasisht, Amanda Saravia-Butler, Samrawit Gebre, Enikő Lázár, Marta Graziano, Solène Frapard, Robert G. Hinshaw, Olaf Bergmann, Deanne M. Taylor, Douglas C. Wallace, Christer Sylvén, Konstantinos Meletis, Julio Saez-Rodriguez, Jonathan M. Galazka, Sylvain V. Costes, Stefania Giacomello